A major mechanism of resistance expressed by MDR pathogens is β-lactamase-mediated degradation of β-lactam antibiotics. Multidrug-resistant (MDR) pathogens pose a significant public health threat. One atom of meropenem is labeled, that of the sulfur atom attached to the pyrroline ring of meropenem ( c). (D) Superposition with the PBP3:meropenem complex (PDB ID 3PBR ). The view is enlarged from panels A and B as the residue F533 position in the apo-PBP3 structure is more distant from the active site. (C) Superposition with the apo-PBP3 (PDB ID 6HZR ). Four moieties of ceftazidime are labeled: amide ( a), dimethyl groups of 2-carboxypropan-2-yl group ( b), methyl substituent of thiazine ring ( c) (methyl group is left after pyridine elimination, see reference 29), and aminothiazole ring ( d). (B) Superposition with the PBP3:ceftazidime complex (PDB ID 3PBO ). Three moieties of carbenicillin are labeled: amide ( a), phenyl ( b), and dimethyl substituents of the thiazolidine ring ( c). All residues labeled are shown in black labels except for Y532 and F533 in the PBP3:WCK 4234 complex, which are in gray as this region adopts a different conformation. Important secondary structure elements β3, β4, and α11 and relevant active site residues are labeled. (A) Superposition with the PBP3:carbenicillin complex (PDB ID 3OCL shown in salmon color). Active site views of superpositions of PBP3:WCK 4234 complex with β-lactam complexes of PBP3 and apo-PBP3.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |